molecular diagnosis of tuberculosis: a new primer design
نویسندگان
چکیده
background nucleic acid based detection of mycobacterium tuberculosis has the potential to improve the analysis of the tuberculosis epidemiology and patient care by increasing the specificity and sensitivity of diagnosis. patients and methods fifty suspected cases were screened for m. tuberculosis by using a new primer sequence and comparing its results with the primer (is6110) most widely used for tuberculosis diagnosis. results the mean age of cases was 33.6±14.3 years. twenty one cases (42%) were positive for m. tuberculosis. maximum positivity was found in sputum (10/19) followed by menstrual blood (4/8), blood (5/15), ascitic fluid (1/2), and pleural fluid (1/5). conclusion the new designed primer was reproducible and in complete concordance with the pcr-results obtained by the standard method using is 6110 insertion sequence in all 50 cases in which both methods were used.
منابع مشابه
Molecular Diagnosis of Tuberculosis
Tuberculosis (TB) is one of the leading causes of adult death in the Asia-Pacific Region, including Indonesia. As an infectious disease caused by Mycobacterium tuberculosis (MTB), TB remains a major public health issue especially in developing nations due to the lack of adequate diagnostic testing facilities. Diagnosis of TB has entered an era of molecular detection that provides faster and mor...
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عنوان ژورنال:
archives of clinical infectious diseasesجلد ۴، شماره ۲، صفحات ۱۰۵-۱۰۸
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